Jobelyn® extends the life span and improves motor function in Drosophila melanogaster exposed to lipopolysaccharide via augmentation of antioxidant status
This present study evaluated its effects on the life span, motor function and changes in oxidative stress parameters as well as acetylcholinesterase activity in Drosophila melanogaster exposed to lipopolysaccharide (LPS).
The flies exposed to LPS (250 μg/kg diet) had reduced life span and elevated oxidative stress when compared with control. However, JB (0.25 and 1.0 mg/kg diet) improved the motor function and also reduced the mortality rate of the flies exposed to LPS. It also restored the cellular antioxidant status and reduced acetylcholinesterase activity, accumulation of hydrogen peroxide as well as nitric oxide in Drosophila fed with LPS. JB also extended the longevity of the flies relative to control. The fndings that JB improves motor function and extended the lifespan of Drosophila flies by boosting the antioxidant status and cholinergic function, suggest it might be helpful in delaying the onset of neuropsychiatric illnesses associated with the aging processes.
The short life span of Drosophila makes it a useful model organism for studying agents capable of promoting longevity and neuro-biology of aging ((Sang and Jackson 2005; Rand 2010). The fly has also been adjudged as a suitable alternative model organism for toxicological investigations especially geno-toxicity (Abolaji et al. 2013).
Jobelyn(R) extends the lifespan of Drosophila flies Figure 1 showed that JB (0.25, 0.5 and 1 mg/g diet) extended the lifespan of the flies above those in the control group. The flies in the control group died earlier relative to those in the intervention groups fed with different doses of JB. Moreover, 45% survival rate was observed in the group of adult flies treated with 0.25 mg/diet of JB as against 10% in the control group (Fig. 1). Survival rate and oxidant molecules in flies exposed to LPS Daily exposure to LPS (250 and 500 μg/kg diet) for 7 days reduced the survival rate of Drosophila flies as presented in Fig. 2. Less than 15% of the flies survived the lethal effect of LPS at a dose of 250 μg/kg diet at day 7 (Fig. 2).
Moreover, LPS significantly (p<0.05) elevated the concentrations of oxidant molecules (hydrogen peroxide and nitrite) accompanied by depletion of catalase (Fig. 2A-C).
The results from this study suggest that JB extended the lifespan and ameliorated LPS-induced locomotor deficit via enhancement of cellular antioxidant defense mechanisms and improved cholinergic neurotransmission in Drosophila melanogaster. Therefore, the consumption of diets fortified with supplements rich in antioxidant activity such as JB should be encouraged, as it might protect the body against the deleterious effects of free radicals and consequently healthy living.
Jobelyn® (JB), a dietary supplement, derived from polyphenol-rich leaf sheath of Sorghum bicolor, has been reported to attenuate sensorimotor deficits and oxidative stress evoked by complete Freund-adjuvant in mice. This present study evaluated its effects on the life span, motor function and changes in oxidative stress parameters as well as acetylcholinesterase activity in Drosophila melanogaster exposed to lipopolysaccharide (LPS). To learn more please click: https://link.springer.com/article/10.1007/s11011-022-00919-4 To learn more about Sorghum bicolor supplement Jobelyn please visit our Google Website: https://health-forever-product-ltd-jobelyn.business.site/